2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. In reverse transcription, the cDNA is synthesized from the mRNA. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Two micrograms of total RNA was reverse-transcribed using an Omniscript Reverse Transcriptase Kit (Qiagen) in a total volume of 20 µl. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Quantiscript 37 NA mRNA Random hexamers Paulin et al. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µL 10 × Buffer RT, 100 µL dNTP Mix (contains 5 mM each dNTP), 1. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Reverse transcriptase is a multifunctional enzyme with three distinct enzymatic activities: an RNA-dependent DNA polymerase, a hybrid-dependent exoribonuclease (RNase H), and a DNA-dependent DNA polymerase. . Qiagen confirmed that such a plasmid is. Kit contents: Qiagen Omniscript RT Kit, 200 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. Kit contents: Qiagen Omniscript RT Kit, 50 rxns,. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. DSL Service Product. Starting material Starting material QIAGEN now offers the first commercially available reverse transcriptases from a new source. The one-step format prevents contamination and allows for higher sensitivity. 1 ml 4. RT-PCR. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. The OneStep Ahead RT Mix contains an RNase inhibitor and a blend of Omniscript® and Sensiscript® reverse transcriptases in combination with an RT-blocker for heat-mediated activation of the reverse-transcription step. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReal-time RT-PCR A OneStep RT-PCR Kitb was used with a 25-#l reaction volume. Additional Information. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). This product is not intended for the diagnosis, prevention, or treatment of a disease. Kim et al. Inquiry. For example, you can create an OmniScript t. The ABScript II Reverse Transcriptase in. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. Product Description. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Global contacts. OmniScript は、お客様がビジネスプロセスを完了するためのガイド付きパスを提供し、シームレスなカスタマーエクスペリエンス (これは 常に 好ましいことです) を実現するため. Workflow for Configuring OmniAnalytics for a Third-Party Analytics. The purification steps can be performed as described in the protocol using Qiagen’s PCR purification kit. Omniscript Reverse Transcriptase - For First-strand cDNA synthesis Two-tube RT-PCR. AIV genomes were amplified from extracted RNA using universal and gene-specific primers with an Omniscript reverse-transcription kit (QIAGEN, Germantown,. 1 ml 1. 381KB. Mix carefully by vortexing for no more than 5 s, and centrifuge briefly to collect residual liquid from the sides of the tube. RT-PCR assays were performed as previously described (Rosciszewski et al. My QIAGEN. CNV qPCR Assays & Arrays;. The sequences of the primers used in. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Subsequent PCR was performed with primers (Sigma-Aldrich) based on the Icelandic rock ptarmigan genome and Taq DNA polymerase (QIAGEN). 0 μg of RNA was reverse-transcribed using the Omniscript RT Kit (Qiagen Inc. g. CNV qPCR Assays & Arrays;. Data Migration with Build Tool. All reactions took place for 1h at 37°C in a final volume of 40 μl containing 1 μg total RNA in the presence of 20 units RNase inhibitor (RNasin® Ribonuclease Inhibitor, Promega), 1 μg random hexamers (Microsynth), 4 μl 10. ZERO BIAS - scores, article reviews, protocol conditions and moreFor 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. 47 / EA. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. CNV qPCR Assays & Arrays;. この例には、再利用可能な. Genomic DNA contamination in RNA samples is effectively. 1 ml RNase-free water. Note: Commercially available RNase inhibitor is commonly supplied at 40 units/µl. It allows for the efficient labeling of a wide range of starting RNA concentrations and increased the signal of our microarrays by 2-5 fold. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. 1 ml RNase-free water 205111 询价For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. This also inactivates the reverse transcriptases. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. For one-step RT-PCR, the optimized Omniscript and Sensiscript blend for the reverse-transcription step further enhances sensitivity. The easy one-tube setup and optimized components enable higher sensitivity. e. 0 µl 5 mM dNTP (final concentration of 500 µM), 2. Isolation and cloning of random PCR products. For each RTase, the reaction conditions and thermal profile followed the recommended protocol issued by the manufacturer (see Protocols in online Data Supplement 1). , SuperScript vs. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Quantitative real-time PCR (qPCR) was performed using the SYBR Premix Ex Taq (TaKaRa, Dalian, China) and the CFX96 TM Real-Time PCR. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Simply add primers for fast and easy cDNA synthesis. A PCR was conducted using standard PCR methods (primers are available on request). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. IL-10 was demonstrated to be critical for suppressing autoimmunity and inflammatory responses ( 40 – 43 ), and whether Th17 express IL-10 is. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription was carried out according to the manufacturer's protocol either with the Omniscript RT (Qiagen) or SuperScript II RT (Invitrogen). Omniscript RT Kit. ·. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR. To minimize methodological errors resulting from. Reverse Transcription Troubleshooting Guide. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. C, Real-time RT-PCR for IL-17 and IL-10 expression in wild-type, DKO, and TKO CD4 + CD25 − T cells after 2 d of culture with anti-CD3 and anti-CD28, combined with IL-6, IL-27, or IL-6 plus IL-27. Amplification and detection were performed with the ABI7500 Sequence Detection System (Applied Biosystems, Foster City, CA). The ABScript II Reverse Transcriptase in the. was reverse transcribed to cDNA using the Omniscript RT kit (Qiagen) and random hexamer primers (Invitrogen; Ta˚strup, Denmark). A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Omniscript, SuperScript III and PowerScript (P < 0. Key words: Breast cancer, Formalin-fixed, Paraffin-embedded tissue, Quantitative RT-PCR,Reverse Transcription was performed using an OmniScript™ RT kit (QIAGEN) according to manufacturer’s instructions and using gene-specific primers to interrogate genes of interest. The Agilent 2100 Bioanalyzer (Agilent Technologies; Kista, Sweden) was used to confirm the integrity of the RNA. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Reverse Transcription Kit (Omniscript® RT Kit) as per the manufacturer's instructions (Qiagen). 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Business Development. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. Format. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Omniscript Reverse Transcription Kit (Qiagen). The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. OmniScript は、お客様がビジネスプロセスを完了するためのガイド付きパスを提供し、シームレスなカスタマーエクスペリエンス (これは 常に 好ましいことです) を実現するための設定可能な方法として機能します。. SYBR Green (DyNAmo SYBR Green qPCR Kit; Finnzymes, Finland) and related accessories to perform real-time RT-qPCR (Opticon2; MJ Research, Watertown,. PCR and RT-PCR are achieved by the use of the hot-start enzyme HotStarTaq Plus DNA Polymerase together with a specialized fast PCR or RT-PCR buffer. Total RNA was reverse-transcribed to cDNA with the use of Qiagen Omniscript RT Kit at 37°C for 60 minutes. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. 1 ml 1. Total RNA was extracted with the use of Qiagen RNeasy Mini Kits for cells and with the use of TRIzol (Invitrogen) for tissue samples according to the manufacturer's protocol. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. CNV qPCR Assays & Arrays;. Pharmaceutical Industry. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. 3. Unit definition: 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR Discover the products and protocols you need Plan your entire experiment based on research interests. Popular answers (1) "If the mRNA has a poly-A 3' tail, then an oligo-dT primer can be used to prime all mRNAs simultaneously. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. EGFP cDNA were obtained with th e other RTases. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. ZERO BIAS - scores, article reviews, protocol conditions and more For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. , M-MuLV or AMV), and the kind of approach used for RT (random hexamers, oligo-dT, or specific. Short cycling steps withoutExplore targets and avenues in their scientific context, find and adjusting products till study i, analyze details and plan follow-up studies – all in GeneGlobe. RT-PCR Assays. This also inactivates the reverse transcriptases. Here, we pres-ent an updated description of current best practices for isolating both mRNA and microRNA from FPE tissues for RT-PCR-based expression profiling. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription from 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. High yields of cDNA 특이성을 높인 참신한 enzyme과 buffer No additional RNase H digestion step required Robust system guarantees consistent results The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. Average lead time: Call for Lead Time. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. DSL Service has an attribute Download Spee d (ATT_RT_DS) and possible values are 7, 12, 20 and 40 Mbps. 1 ml RNase-free water 205111 询价 The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. Omniscript RT Kit. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRLaunching an OmniScript with an OS Action in a Community; Launching an OmniScript from a Layout Action; Launching an OmniScript with an OS Action in Lightning; Launching an LWC OmniScript in Lightning; Launching an OmniScript in Lightning; Launching an OmniScript From the Action Toolbar; Launching an LWC OmniScript from an OS. 1 ml 4. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Omniscript is a confident hand-lettered script without self-conscious style or idiosyncrasy. Components. 4 Omniscript Reverse Transcriptase Handbook 04/99 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog No. Workflow Configurator. 1). Omniscript is designed for reverse transcription of RNA amounts greater than 50 ng, andFor reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRExperimental validation. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Omniscript RT Kit. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich. , Valencia, CA). Differences Between OmniStudio and OmniStudio for Vlocity. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spreads, leading to a pandemic infection. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Semiquantitative RT-PCR. 1x Buffer RT (dilute an aliquot of 10x Buffer RT accordingly using the RNase -free water supplied). Sample to Insight__ Quick-Start Protocol March 2016 Omniscript® Reverse Transcription Kit The Omniscript RT Kit (cat. For reverse transcription of 50 ng to 2 μg RNA per reactivity for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROverall, my experience with Qiagen’s OmniScript RT Kit has been good. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Product tracking (in bp) are shown about the right of the panel (lane 9). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. # 18064 and 18080, respectively), and Omniscript® and Sensiscript® (Qiagen Cat. For reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCR Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ngから2 µgのRNA量を用いた逆転写用に特別にデザインされています。 RNAへのアフィニティーが高いOmniscript Reverse Transcriptaseは、他の逆転写酵素と比較して非常に優れた性能を示し、低いコピー数の. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. 1 ml 1. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. CNV qPCR Assays & Arrays; Microbial DNA qPCR Assays & Panels For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Qiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. (B) Plasmid map of the predicted Omniscript RT Kit expression plasmid which was identified as the source of the EIAV pol. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Kit Quick-Start Protocol (EN) Print Bookmark Share pdf 56KB English Format File size Language Download Get Adobe Reader Contact QIAGEN . Media Inquiries. The assays were run under the following conditions. Omniscript Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRBalanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. 00. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRNxtScript 2G RT is the latest member of the family and pushes the temperature of the RT step to new levels: no loss of activity even at 70°C. Heat-mediated activation of these enzymes enables reaction setup at room temperature. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 1 ml 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Omniscript Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus (AMV). First Strand kit)? What is recommended? We have had a good experience with both types of Reverse Transcriptases, e. ers and the same RT kit. Your reverse transcription may not be optimal. pdf. less than 500 ng total RNA). Matrix assay. The ideal reverse transcriptase is robust (highly active under a variety of conditions) and converts all primed RNA within a sample to cDNA, regardless of its abundance, length or secondary structure. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription is described based on the Omniscript ® Reverse Transcription Kit (Qiagen). . The QIAGEN One-Step Ahead RT-PCR Kit contains a blend of Sensiscript and Omniscript Reverse Transcriptases, a well-balanced combination of Taq DNA Polymerases and a proofreading enzyme. Four weights together with monospaced numerals and math symbols make it ideal for. Omniscript Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus. 次の画像で. Download. Omniscript and Sensiscript QuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. This kit's protocol is simple and suitable for high throughput. Data Migration with Build Tool. ZERO BIAS - scores, article reviews, protocol conditions and morePeripheral blood of individual 4 was collected into PAXgene Blood RNA tubes (PreAnalytiX), and RNA was isolated using the PAXgene Blood RNA Kit (Qiagen). There-fore, 2,500 copies (1 fg) was chosen as the relatively small. Total RNA was isolated from drug-treated cells with TRIzol (Invitrogen) and then reverse-transcribed to cDNA using the Omniscript RT kit (Qiagen, Valencia, CA). 1 ml 4. Bioz Stars score: 86/100, based on 1 PubMed citations. CNV qPCR Assays & Arrays;. Popular answers (1) "If the mRNA has a poly-A 3' tail, then an oligo-dT primer can be used to prime all mRNAs simultaneously. The rela‑ tive gene expression levels were normalized to GAPDH and calculated using the 2‑ΔΔCq method. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript® Reverse TranscriptionFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR3 min read. . Omniscript RT Kit. . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Reverse transcription was applied with Omniscript RT Kit following manufacturer's protocol (Qiagen). The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 1fg/µl). Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. Real-time PCR analysisReverse Transcriptase was performed using Omniscript RT kit (Qiagen, Valencia, CA, #205113) according to manufacturer’s protocol. Bioz Stars score: 96/100, based on 1 PubMed citations. 1 ml 4. It allows for the efficient labeling of a wide range of starting RNA concentrations and. All components that are required for fast and efficient reverse transcription are provided with the QuantiTect. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. At this reaction temperature, common restrictions of RT-PCR are removed, simplifying assay design and eliminating compromises. Reverse transcription is a powerful tool for creating complementary DNA (cDNA) from RNA, which can then be used in a variety of downstream applications for studying RNA. 00. Serial analysis of BCR-ABL mRNA levels by real-time quantitative PCR (RQ-PCR) accurately reflects the level of leukemic inhibition induced by therapy and provides. Omniscript reverse transcriptase (QIAGEN) using random hexamers and oligo dT and. It is crucial to recognize and prevent potential issues with cDNA synthesis in order to maintain the reliability of experimental. SuperScript II, AMV-RT, Transcriptor RT, M-MuLV RT, M-MLV RT, Omniscript, DyNAmo, Statascript: Covariances of the RT efficiency were driven by target gene or total RNA concentration: Enzyme differences less important than diversity in gene-specific RT reproducibility: Bustin et al. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The QIAGEN OneStep RT-PCR Enzyme Mix contains a specially formulated enzyme blend for both reverse transcription and PCR. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Balanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. Vlocity Cards Framework. これにより、特定の取引先責任者や取引先の項目を表示するなどの一般的な機能を持つ小さな「子」 OmniScript を作成することができます。. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. Global Contacts. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. DSL Modem Product. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. RNA was the same set of samples used for Illumina GAII sequencing analysis. , Valencia, CA). Please see the Omniscript Reverse Transcription Handbook for protocols. The Omniscript RT Kit is intended for molecular biology applications. 1 ml RNase-free water 205111 询价Currently, almost all single-cell transcriptomic protocols rely on reverse transcription (RT). For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. Differences Between OmniStudio and OmniStudio for Vlocity. During reverse transcription, chemically modified HotStarTaq DNA Polymerase is completely inactive and does not interfere with the reverse-transcriptase reaction. RT-PCR; PCR for Difficult Targets; Fast PCR; Long PCR; Direct PCR; High Fidelity PCR; Hot Start PCR; Real-Time PCR; Routine PCR ReagentOmniscript Rt Kit, supplied by Qiagen, used in various techniques. It uses PrimeScript Reverse Transcriptase, which features excellent extension. Tie a Picklist to Salesforce. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR OmniScripts. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. We compared these five RTs for their utility in detecting trace transcripts by real-time PCR, ability to transcribe a long RNA and sensitivity. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. A unique enzyme combination and specially developed reaction buffer ensure efficient, highly specific reverse transcription and PCR in one tube, without the need for. OmniScripts allow you to craft dynamic customer. RNA from Svalbard ptarmigan brain samples was extracted using TriReagent (Sigma-Aldrich) and the extracted RNA was converted into cDNA using the Omniscript RT kit from QIAGEN. 95°C after the reverse transcription step. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. , 2018). ,. Omniscript and Sensiscript QuantiFast RT Mix contains an optimized Omniscript and Sensiscript blend. It uses PrimeScript Reverse Transcriptase, which features excellent extension. Both biological and technical triplicates were run. PCR was performed with HotStarTaq PCR Kit (Qiagen). First-strand cDNA synthesis was done using the Omniscript RT kit (Qiagen, Valencia, CA) as recommended by the manufacturer. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. OmniStudio Standard. RNA was transcribed into cDNA using the Omniscript RT kit (Qiagen). Please see the Omniscript Reverse Transcription Handbook for protocols. Please see the Omniscript Reverse Transcription Handbook for protocols. Relative quantification of mRNA levels by RT-qPCR was performed using a SYBR Green PCR kit (Qiagen Inc. Reactions were incubated at 65°C for 5 min, then 100 U RT enzyme was added and incubated at 37°C for 60 min and 75°C for 5 min. 1 ml 1. OmniScript を使用しましょう。. QIAsphere Digital Connectivity. 2800. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. DSL Modem has an attribute Grade (ATT_RT_GR) and possible values are Good, Better and Best. CNV qPCR Assays & Arrays;. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. , GC-rich. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 × 150 µL 10 × Buffer RT, 4 × 100 µL dNTP Mix (contains 5 mM each dNTP), 4 × 1. RT-PCR Analysis . Probably the most important being that the OmniScript RT allows for the labeling of even very small amounts of RNA (i. Reverse transcription (RT) is a critical step in a real-time PCR. Quantitative real-time PCR was performed on a QuantStudio 6 Pro real-time PCR system and Taqman Universal PCR Master Mix according to the manufacturer instructions. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. For reverse transcription from 50 ng to 2 μg RNA per reaction for end-point PCRAdditional Information. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript RT Kit. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRRT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. ) to obtain cDNA. 1 mL RNase-free. QIAstock. Commercial Partner and Distributor Solutions. Reverse transcriptase is a multifunctional enzyme with three distinct enzymatic. Reactions containing 2. EGFP cDNA were obtained with th e other RTases. Related products . Spoorstraat 50 5911 KJ Venlo The Netherlands Orders 0800-79612 Fax 0800-79611 Technical 0800-79556 Canada QIAGEN Inc. USD $1207. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. # 205113 and 205213, respectively) reverse transcriptases. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. This enzyme combination provides highly efficient and sensitive reverse transcription over a wide range of RNA templateLightning Experience. 05). Omniscript RT Kit. In order to measure this difference on a large spectrum of background RNA, given that DNA and RNA concentrations are important influencing factors [ 8 – 10 ], the study used total RNA extracted from the same testis tissue (Methods). High Ca2+ stimulated the upregulation (as assessed by RT-PCR) and the secretion of Wnt5a (assessed by Western blot), a. Omniscript RT Kit (200) by Qiagen. icon_0011_idea-s. nos. Actin and CD30 cDNA transcripts were quantified using AB&I TaqMan primer and probe sets (Life Technologies) on a Bio-Rad CFX96 real-time. Language. Omniscript is designed for reverseFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThis study provides a complex overview of the influence of elements such as RT systems, qRTPCR chemistry, amount of background RNA, and transcript abundance on the efficiency of qRT-PCR. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 x 150 µl 10x Buffer RT, 4 x 100 µl dNTP Mix (contains 5 mM each dNTP), 4 x 1. 1 ml RNase-free water 205111 询价 Reverse transcription and quantitative real-time PCR (RT-qPCR). RT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. 2 μg of total RNA was used for reverse transcriptase synthesis using the. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Principle Omniscript Reverse Transcriptase (RT) has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA. It relies on the principle of reverse transcription which is a process ‘reverse’ to the transcription. Total RNA was also extracted from peripheral blood of the patient using the RNeasy Mini Kit (Qiagen Inc. There are several commercially available RT kits which differ mainly in the kind of enzyme they use, bacterial (rTth) or viral origin (e. g. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. Add 2 μl of Buffer RT (component of Omniscript RT Kit), 2 μl of dNTP Mix (component of. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The cDNA was generated using the Omniscript RT kit (Qiagen) with random hexamers (Roche Products, United States). The reverse transcription (RT) step conditions for all primer sets were 30 min at 50 C and 15 min at 95 C. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Enzyme activity: Reverse tran…The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. g. Direct quantification of six different targets in human total RNA using GoTaq® 2-Step RT-qPCR System. Reverse transcription reaction conditions RT kits used in this experiment were chosen to represent the most commonly used enzymes in the field (Fig. PCR reactions were performed to determine Clk 1 mRNA levels using JumpStart Ready-Start amplification mix (Sigma) and previously published primers. 1. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. QIAGEN Subsidiaries. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. USD $1207. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Mar 15, 2021. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. Omniscript RT system, whereas substantial copies of. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Content. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFAQ. The one-step format prevents contamination and allows for higher sensitivity. Background Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. 1 ml 4. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. The cDNA was then synthesized with an Omniscript Reverse Transcription (RT) Kit (Qiagen) or Moloney Murine Leukemia Virus Reverse Transcriptase Reverse Transcriptase (M-MLV RT). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 31 Australia QIAGEN Pty Ltd PO Box 641 DONCASTER VIC 3108 Orders 03-9840-9800 Fax 03-9840-9888 Technical 1-800-243-066 Belgium QIAGEN Benelux B. Briefly, RNA was isolated using the RNAeasy Mini kit (Qiagen, Germany). To understand the role of the colonic extracellular calcium-sensing receptor (CaSR) in calcium chemoprotection against colon cancer, we activated the CaSR with 5 mM Ca2+ on HT-29 cells, an adenocarcinoma cell line. The enzyme mix is specially formulated for both reverse transcription and PCR. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor RNA, is there a difference in selecting RT enzymes with and without RNase H (e. coli. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR(RT-PCR) RNA was extracted from cultured macrophages with RNeasy Mini Kit (Qiagen, Hilden, Germany) and made into cDNA with Omniscript RT Kit (Qiagen) according to the manufac-turer’s instructions. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRLevels of IL-6 mRNA in cells were evaluated by real-time reverse transcription and Omniscript Reverse Transcriptase Journal of Neuroinflammation.